Part I (Prof. Panke)
- What’s an RBS and a rho independent termination sequence and what are the macromolecules involved.
- If you have a gene in an ORF of a second gene can you change the expression of the first gene without moving it.
- Describe the lac system with all its components in its biological form.
- The lac system is an all or nothing system, describe what that means. Design a simple experiment to showcase with E.coli with GFP controlled by Placz to show this all or nothing behavior. If you add glucose to your experiment does anything change?
- What other than all or nothing can you create with regulation and how would you modify the lac system to exhibit this behavior?
- Design a (biological) bandpass filter with E.coli that sends out AHL. It creates a band on a petri dish.
- What would you change to increase the distance to the inner circle?
- What would you change to decrease the distance to the outer circle?
- How do you design another bandpass with another fluorescent protein that is further away from the centre without the two bandpasses being able to overlap?
Part II (Prof. Stelling)
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